Peptides and cyanine dyes. 2. Covalent labelling of amino acids and peptides by means of the reaction of pyrylium cyanine dyes with amines

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Photoswitching Mechanism of Cyanine Dyes

Cyanine dyes have been shown to undergo reversible photoswitching, where the fluorophore can be switched between a fluorescent state and a dark state upon illumination at different wavelengths. The photochemical mechanism by which switching occurs has yet to be elucidated. In this study, we have determined the mechanism of photoswitching by characterizing the kinetics of dark state formation an...

متن کامل

Development of photostable near-infrared cyanine dyes.

With the emerging interest in optical in vivo imaging, there is an increasing demand of photostable near-infrared (NIR) dyes. Herein we report the rational design of an amine tricarbocyanine structure with improved photostability (CyNA) and its combinatorial derivatization to render CyNA-414 as a NIR-fluorescent dye with stronger emission intensity and higher photostability than the NIR standar...

متن کامل

Enhanced brightness and photostability of cyanine dyes by supramolecular containment

Ultrasensitive detection and real-time monitoring of biological processes can benefit significantly from the improved brightness and photostability of the popular organic dyes such as cyanines. Here, using a model cyanine dye, Cy3, we demonstrate that brightness and photostability of the dye is significantly altered when trapped in a molecular container, e.g. cucurbit[n]urils (CB[n]) and cyclod...

متن کامل

Heterobifunctional Dyes: Highly Fluorescent Linkers Based on Cyanine Dyes

Herein, we present a new synthetic route to cyanine-based heterobifunctional dyes and their application as fluorescent linkers between polymers and biomolecules. The synthesized compounds, designed in the visible spectral range, are equipped with two different reactive groups for highly selective conjugation under physiological conditions. By applying indolenine precursors with functionalized b...

متن کامل

Amino Acids, Amines and Peptides of Ram

Epididymal semen was obtained from rams with a fistula present in the vas deferens (Tadmor & Schindler, 1966). It was deproteinized immediately, and the protein-free clear supernatant evaporated to dryness in vacuo at a temperature not exceeding 55\s=deg\C. The residue was taken up with about 10 ml water, and the solution thus obtained used for determination of both amines and amino acids. For ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Biopolymers and Cell

سال: 2001

ISSN: 0233-7657,1993-6842

DOI: 10.7124/bc.0005a1